Journal: JCI insight

Article Title: PCYT2 inhibits epithelial-to-mesenchymal transition in colorectal cancer by elevating YAP1 phosphorylation.

doi: 10.1172/jci.insight.178823

Figure Lengend Snippet: Figure 4. PCYT2 affects the phosphorylation levels of YAP1 and changes the proportion of YAP1 in 778

Article Snippet: Cell extracts were 520 prepared by mechanical sonication, and protein–DNA complexes were immunoprecipitated 521 using anti-YAP1 antibodies (Cell Signaling Technology, 14074T).

Techniques: Phospho-proteomics

Journal: JCI insight

Article Title: PCYT2 inhibits epithelial-to-mesenchymal transition in colorectal cancer by elevating YAP1 phosphorylation.

doi: 10.1172/jci.insight.178823

Figure Lengend Snippet: Figure 5. PCYT2 regulates EMT in colorectal cancer via YAP1 translocation induced by PEBP1. (A 802

Article Snippet: Cell extracts were 520 prepared by mechanical sonication, and protein–DNA complexes were immunoprecipitated 521 using anti-YAP1 antibodies (Cell Signaling Technology, 14074T).

Techniques: Translocation Assay

Journal: JCI insight

Article Title: PCYT2 inhibits epithelial-to-mesenchymal transition in colorectal cancer by elevating YAP1 phosphorylation.

doi: 10.1172/jci.insight.178823

Figure Lengend Snippet: Figure 6. PPP2R1A interacts with YAP1 and PEBP1. (A) Detection of the interaction between PEBP1 820

Article Snippet: Cell extracts were 520 prepared by mechanical sonication, and protein–DNA complexes were immunoprecipitated 521 using anti-YAP1 antibodies (Cell Signaling Technology, 14074T).

Techniques:

Journal: Developmental cell

Article Title: Yorkie growth promoting activity is limited by Atg1-mediated phosphorylation

doi: 10.1016/j.devcel.2020.01.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-pS97-Yorkie (rabbit polyclonal) , This work; Genemed Synthesis , N/A.

Techniques: Recombinant, SYBR Green Assay, Plasmid Preparation, Protease Inhibitor, Transfection, Chromatography, Electron Microscopy, Membrane, Reverse Transcription, Software

Journal: Translational Lung Cancer Research

Article Title: Targeting PKCι-PAK1 in EGFR-mutation positive non-small cell lung cancer

doi: 10.21037/tlcr.2019.08.25

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: Mouse anti-YAP1 , 1:1,000 , Cell Signaling (#12395).

Techniques:

Journal: OncoTargets and therapy

Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis

doi: 10.2147/OTT.S292287

Figure Lengend Snippet: Primer Sequences

Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); rabbit anti-human Yap1 polyclonal antibody (1:2000, Proteintech, Chicago, Illinois, USA); rabbit anti-human GAPDH monoclonal antibody (1:3000, Proteintech, Chicago, Illinois, USA) were used as internal reference antibodies.

Techniques: Sequencing

Journal: OncoTargets and therapy

Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis

doi: 10.2147/OTT.S292287

Figure Lengend Snippet: ANXA1 and Yap1 are involved in many of the same biological processes (BP) and play an important role in LN metastasis of HSCC. ( A ) BP GO terms of ANXA1; ( B ) KEGG pathway enrichment analysis. ( C ) Some of the BP GO of Yap1; ( D ) PPI network of genes involved in the cancer biological processes with both ANXA1 and Yap1.

Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); rabbit anti-human Yap1 polyclonal antibody (1:2000, Proteintech, Chicago, Illinois, USA); rabbit anti-human GAPDH monoclonal antibody (1:3000, Proteintech, Chicago, Illinois, USA) were used as internal reference antibodies.

Techniques:

Journal: OncoTargets and therapy

Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis

doi: 10.2147/OTT.S292287

Figure Lengend Snippet: Down-regulation of ANXA1 decreases Yap1 protein levels. ( A and B ) The effects of ANXA1 silencing on Yap1 mRNA and protein levels were quantified by qRT-PCR and Western blotting; ( C ) mRNA expression levels of ANXA1 in HSCC tissues (10 samples from patients with LN metastasis,10 samples from patients without LN metastasis); ( D ) Yap1 protein expression was detected by Western blotting in HSCC patients (left) and quantitated relative to GAPDH (6 samples from patients with LN metastasis patients, 6 samples from patients without LN metastasis); ( E ) Pearson correlation analysis of ANXA1 and Yap1 expression levels. The expression of ANXA1 mRNA is used as the X-axis after logarithmic transformation. Similarly, Yap1 is represented on the Y-axis.

Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); rabbit anti-human Yap1 polyclonal antibody (1:2000, Proteintech, Chicago, Illinois, USA); rabbit anti-human GAPDH monoclonal antibody (1:3000, Proteintech, Chicago, Illinois, USA) were used as internal reference antibodies.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Transformation Assay

Journal: OncoTargets and therapy

Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis

doi: 10.2147/OTT.S292287

Figure Lengend Snippet: Yap1 over-expression reverses the effects of ANXA1 silencing on FaDu cells. ( A ) The transfection efficiency of lentivirus was observed by fluorescence microscope. GFP and cherry represent the fluorescence intensity of sh-ANXA1 and oe-Yap1, respectively. Fluorescence micrograph (100 ×); ( B ) qRT-PCR was used to detect the mRNA expression levels of ANXA1 and Yap1. GAPDH was used as reference; ( C ) The expression of ANXA1 and Yap1 protein levels were detected by WB. GAPDH was used as internal control; ( D ) CCK8 staining assay was used to detect the cell proliferation activity for 4 days.; ( E ) Colony formation assay was used to detect the cell proliferation; ( F ) Transwell assay was used to determine the migration and invasion abilities of FaDu cells (200 × magnification; image acquired after 24 h); ( G and H ). Three days after transfection of Yap1, the apoptosis rate ( G ) and cell cycle status ( H ) of FaDu cells were assessed by FCM. Data are presented as mean ± SD of triplicates.

Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); rabbit anti-human Yap1 polyclonal antibody (1:2000, Proteintech, Chicago, Illinois, USA); rabbit anti-human GAPDH monoclonal antibody (1:3000, Proteintech, Chicago, Illinois, USA) were used as internal reference antibodies.

Techniques: Over Expression, Transfection, Fluorescence, Microscopy, Quantitative RT-PCR, Expressing, Control, Staining, Activity Assay, Colony Assay, Transwell Assay, Migration